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		<h1><a href="index.php">PRAPI</a></h1>
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			<h2>Welcome to use PRAPI</h2>
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		  	PRAPI is  a one-stop solution for Iso-Seq analysis of analyze <a href="example.php#sec1">alternative transcription initiation (ATI), alternative splicing (AS), alternative cleavage and polyadenylation (APA)</a>, <a href="example.php#sec2">natural antisense transcripts (NAT)</a>, and <a href="example.php#sec9">circular RNAs (circRNAs)</a> comprehensively. PRAPI is capable of combining Iso-Seq full-length isoforms with short read data, such as RNA-Seq or polyadenylation site sequencing (PAS-seq) for differential expression analysis of <a href="example.php#sec6">NAT</a>, <a href="example.php#sec4">AS</a>, <a href="example.php#sec3">APA</a> and <a href="example.php#sec5">circRNAs</a>. Furthermore, PRAPI can annotate <a href="example.php#sec7">new genes</a> and <a href="example.php#sec8">correct mis-annotated genes</a> when gene annotation is available. Finally, PRAPI generates high-quality vector graphics to visualize and highlight the Iso-Seq results.
		  	
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		  	<a href="">(A)</a>. The workflow of PRAPI for identification and quantitative analysis of post-transcriptional regulation.
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		  	<a href="">(B)</a>. Snapshot of post-transcriptional regulation analysis. The top track represents Iso-Seq alignment. The middle track shows the wiggle from RNA-Seq and PAS-seq. The bottom track shows the alternative back-splicing in circRNAs which span the region of AS and APA.
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		  Citing PRAPI:
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			Gao Y, Wang H, Zhang H, Wang Y, Chen J, Gu L, (2017), PRAPI: post-transcriptional regulation analysis pipeline for Iso-Seq.<i>Bioinformatics,Bioinformatics</i>, btx830,https://doi.org/10.1093/bioinformatics/btx830
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